EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.8 | wild type and mutant enzymes are expressed in HEK-293T/17 cells | Homo sapiens |
3.1.1.84 | wild type and mutant enzymes are expressed in HEK-293T/17 cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.8 | A199S/F227A/S287G/A328W/E441D | the mutant has significantly lower kcat and KM values against acetylcholine than the wild type enzyme | Homo sapiens |
3.1.1.8 | A199S/F227A/S287G/A328W/Y332G/E441D | the mutant has significantly lower kcat and KM values against acetylcholine than the wild type enzyme | Homo sapiens |
3.1.1.8 | A199S/S287G/A328W/Y332G | the mutant has significantly lower kcat and KM values against acetylcholine than the wild type enzyme | Homo sapiens |
3.1.1.84 | A199S/F227A/S287G/A328W/E441D | the mutant shows 1730fold improved (-)-cocaine-hydrolyzing activity compared to the wild type enzyme | Homo sapiens |
3.1.1.84 | A199S/F227A/S287G/A328W/Y332G/E441D | the mutant shows 1390fold improved (-)-cocaine-hydrolyzing activity compared to the wild type enzyme | Homo sapiens |
3.1.1.84 | A199S/S287G/A328W/Y332G | the mutant shows 1080fold improved (-)-cocaine-hydrolyzing activity compared to the wild type enzyme | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.8 | 0.027 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.8 | 0.036 | - |
Acetylcholine | mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.8 | 0.075 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.8 | 0.148 | - |
Acetylcholine | wild type enzyme, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 0.0011 | - |
(-)-cocaine | mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 0.0031 | - |
(-)-cocaine | mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 0.0035 | - |
(-)-cocaine | mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 0.0045 | - |
(-)-cocaine | wild type enzyme, pH not specified in the publication, at 25°C | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.8 | Homo sapiens | - |
- |
- |
3.1.1.84 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.8 | QFF resin anion-exchange chromatography and Hypatite C column chromatography | Homo sapiens |
3.1.1.84 | QFF resin anion-exchange chromatography and Hypatite C column chromatography | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.8 | blood plasma | - |
Homo sapiens | - |
3.1.1.84 | blood plasma | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.8 | acetylcholine + H2O | - |
Homo sapiens | choline + acetate | - |
? | |
3.1.1.84 | (-)-cocaine + H2O | - |
Homo sapiens | benzoic acid + methyl (1R,2R,3S,5S)-3-hydroxy-8-methyl-8-azabicyclo[3.2.1]-octane-2-carboxylate | i.e. ecgonine methyl ester | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.8 | BChE | - |
Homo sapiens |
3.1.1.8 | butyrylcholinesterase | - |
Homo sapiens |
3.1.1.84 | BChE | - |
Homo sapiens |
3.1.1.84 | butyrylcholinesterase | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.8 | 125 | - |
Acetylcholine | mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.8 | 230 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.8 | 243 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.8 | 1433 | - |
Acetylcholine | wild type enzyme, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 0.002 | - |
(-)-cocaine | wild type enzyme, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 28.83 | - |
(-)-cocaine | mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 51 | - |
(-)-cocaine | mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 73.83 | - |
(-)-cocaine | mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.8 | 3000 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.8 | 3500 | - |
Acetylcholine | mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.8 | 9000 | - |
Acetylcholine | mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.8 | 9667 | - |
Acetylcholine | wild type enzyme, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 15.17 | - |
(-)-cocaine | wild type enzyme, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 16500 | - |
(-)-cocaine | mutant enzyme A199S/S287G/A328W/Y332G, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 21670 | - |
(-)-cocaine | mutant enzyme A199S/F227A/S287G/A328W/Y332G/E441D, pH not specified in the publication, at 25°C | Homo sapiens | |
3.1.1.84 | 26670 | - |
(-)-cocaine | mutant enzyme A199S/F227A/S287G/A328W/E441D, pH not specified in the publication, at 25°C | Homo sapiens |